ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (3): 375-379.doi: 10.11843/j.issn.0366-6964.2014.03.005

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The Transcription of S100A12 Gene Negatively Regulated by Sox-5 in Pig

XU Jing,TANG Juan,LIU Ying,ZHAO Shu-hong,LI Xin-yun*   

  1.  (Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Huazhong Agricultural University,Wuhan 430070, China)
  • Received:2013-07-05 Online:2014-03-23 Published:2014-03-23

Abstract:

This study aimed to investigate the negative transcriptional factors and their binding motifs in the promoter region of porcine S100A12 gene.The promoter transcriptional activity of the regions,which contains -1 013-+598,-931-+598,-830-+598,-711-+598 and -590-+598 were detected by dual luciferase assay system.Furthermore,the transcriptional activity and the transcriptional factors’ binding sites located in the -1 013--931 region was deeply investigated through DNA deletion and mutation analyses.Porcine S100A12 promoter activity decreased significantly(P<0.05)when the region -1 013--931 was deleted.Bioinformatics analyses results showed that transcriptional factor Sox-5 and GATA-1 binding sites located in this region.The promoter activity was significantly increased only when the binding site of Sox-5 was mutant or deleted.The results indicate that the expression of S100A12 gene is negatively regulated by the transcriptional factor Sox-5.

CLC Number: